Connections to all major national and international destinations are available from Memphis International Airport located within 45 minutes from Holly Springs. Memphis is a national hub for Northwest Airlines and world headquarters for FedEx. Memphis is one of the busiest airports in the nation and the world's largest cargo airport.

Local air service is available at the Holly Springs-Marshall County Airport which is managed by the Industrial Development Authority. The airport has a 3,200 foot long, 60 foot wide runway. The FBO is operated by John Jewell Aircraft which provides engine and airframe repairs